Agarose (Low Electroendosmosis) For Electrophoresis

Specification of Agarose (Low Electroendosmosis) For Electrophoresis

SELL AGAROSE ELECTROPORESIS GEL

Agarose is a polysaccharide, generally extracted from certain red seaweed. This is a linear polymer consisting of a repeat unit of agarobiose, which is a disaccharide consisting of D -galactose and 3,6-anhydro-L-galactopranranose. Agarose is one of the two main components of agar, and is purified from agar by removing the other agar component, agaropectin.

Its properties

- Gel temperature 35-42 ° C and melting temperature 85-95 ° C.

- Low melting agarose and low gel made through chemical modification are also available.

- Agarose gel has a large pore size and good gel strength, making it suitable as an anticonvection medium for electrophoresis of DNA and large protein molecules.

- 1% gel pore size has been estimated from 100 nm to 200-500 nm and the strength of the gel allows a thin gel of 0.15% to form plates for gel electrophoresis

Agarose is often used in molecular biology for the separation of large molecules, especially DNA, by electrophoresis. Agarose gel sheets (usually 0.7 - 2%) for electrophoresis are prepared by pouring a warm, liquid solution into the mold. A variety of different agaroses of various molecular weights and properties are commercially available for this purpose. Agarose can also be formed into beads and used in a number of chromatographic methods for protein purification.

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