Specification of Agarose (Low Electroendosmosis) For Electrophoresis
SELL AGAROSE ELECTROPORESIS GEL
Agarose is a polysaccharide, generally extracted from
certain red seaweed. This is a linear polymer consisting of a repeat unit of
agarobiose, which is a disaccharide consisting of D -galactose and
3,6-anhydro-L-galactopranranose. Agarose is one of the two main components of
agar, and is purified from agar by removing the other agar component,
agaropectin.
Its properties
- Gel temperature 35-42 ° C and melting temperature
85-95 ° C.
- Low melting agarose and low gel made through
chemical modification are also available.
- Agarose gel has a large pore size and good gel
strength, making it suitable as an anticonvection medium for electrophoresis of
DNA and large protein molecules.
- 1% gel pore size has been estimated from 100 nm to
200-500 nm and the strength of the gel allows a thin gel of 0.15% to form
plates for gel electrophoresis
Agarose is often used in molecular biology for the
separation of large molecules, especially DNA, by electrophoresis. Agarose gel
sheets (usually 0.7 - 2%) for electrophoresis are prepared by pouring a warm,
liquid solution into the mold. A variety of different agaroses of various
molecular weights and properties are commercially available for this purpose.
Agarose can also be formed into beads and used in a number of chromatographic
methods for protein purification.
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